Scientifica implantology

Minerva Stomatologica, 58:317-30, 2009;

The in-vitro experimental protocol verifi ed the capacity of laser treatment to remove from various implant surfaces the bacterial load induced to simulate the presence of perimplantitis. The results confi rm the effectiveness of laser treatment; however, the authors recommend repeated applications.

Clinical Oral Implant Research 21: 756–765, 2010

The in-vitro behaviour of the Sweden & Martina surfaces in contact with human osteoblastic cell culture demonstrates that the roughening of the surface offers greater contact area and interconnection with the bone interface.

Clinical Oral Implant Research, 21(10): 1172, 2010

The condition that makes an in-vitro experimentation correct in biochemical terms essentially consists of being able to supply the selected cell lines with the factors that govern the same in-vivo cell processes, stimulating the cells to differentiate themselves in a correct manner. Therefore, this study focused on testing the biocompatibility of a new experimental surface for intraosseous implants that combines a commercially pure titanium surface or a surface sandblasted and acid-etched with phosphoserinepoly( lysine) dendrimers, analysing the effects of this bio-coating on cell differentiation and on the activation of pathways that mediate cell differentiation. For this purpose, the murine MC3T3 osteoblastic cell line and primary murine marrow cells were selected and positioned in a culture medium enriched with ascorbic acid on disks of smooth titanium, sandblasted and acid-etched (SA) and sandblasted and acid-etched with bio-coating (cSA) for a duration of six days.
The cell attachment was measured with MTT assay and the gene expression was verifi ed with Real time PCR. To study the activation of the WNT signalling pathway, critical for osteoblastic commitment, we transfected the C2C12 mesenchymal cells on titanium surfaces with a vector system expressing the firefly luciferase reporter gene under the control of a promoter sequence activated by TCF beta-catenin and a control vector expressing Renilla Luciferase. The results of the above tests show that the bio-coating improved cell attachment at 48 hours and increased the transcription levels of alkaline phosphatise and osteocalcin, two osteoblastic phenotype markers after 6 days of culture. The Osterix mRNA levels, an early osteoblastic marker, decreased between the 3rd and 6th day in all groups, although on the 6th day
these levels were still higher on the cSA surface compared to the other two groups. The osteoprotegerin expression, a protein that antagonises osteoclast formation, was also higher in cells that grew on the cSA surface after 3 days and also after 6 days of culture. In the same way, on the 6th day, the mRNA revels of another target gene of the same pathway, WISP-2 and beta-catenin, the transcription factor of this pathway, were signifi cantly higher in this group. At this point, the C1C12 transfected cells were stimulated with a WNT3a recombined soluble protein and there was a higher increase in the activity of luciferase in cells attached to the cSA surface. The results of this study showed that this new bio-coating improves cell attachment and promotes the expression of a mature osteoblast phenotype, stimulating at the same time, activation of the WNT signalling pathway and increasing its activation by means of exogenous stimuli.

This in-vitro study evaluated the behaviour of cells in contact with a new experimental surface coated with phosphoserine-poly(lysine) dendrimers, and verified its positive stimuli to cell growth and attachment.

Journal of Periodontology, 82 (8): 1169-1177, 2011

The authors conducted in-vitro tests on the use of the Erbium YAG laser for treating different implant surfaces inoculated with different bacterial strains to simulate the conditions of peri-implantitis. They verified that the power of this laser ray is capable of modifying the surface morphology, thus influencing the process of cell multiplication and attachment of the newly formed bone.

Dental Material Journal, 30: 183-192, 2011

This study once again reconfirms how surface roughening positively influences the immunohistochemical parameters of neo-osteogenesis.

Clinical Oral Implant Research, 22(9), 2011: 1071

This experimental study evaluated how the different surface morphologies were able to favourably modulate the gene expression responsible for osteogenesis.

Clinical Oral Implant Research, 22(9), 2011: 1082-3

In the first hours of contact with the biological fluids, a smooth titanium surface treated with Plasma Cleaning favoured the adhesion of in-vivo fibroblasts. The authors suggest that this protocol could potentially bolster the attachment of mucous to the implant posts.

Clin Oral Implants Res. 2016 Jun 28. doi: 10.1111/clr.12913. [Epub ahead of print]

This study analyzed the different sequential healing phases of cortical bone vs bone marrow after 5, 10, 20 and 30 days. In cortical bone, the replacement of old bone with new bone was closely linked to remodelling of the individual BMUs, while in the bone marrow, new bone developed in very dense successive layers.